Amplify, Amplify: Shotgun Proteomics Boosts the Signal for Biomarker Discovery

Amplify, Amplify: Shotgun Proteomics Boosts the Signal for Biomarker Discovery

Shotgun Proteomics and Biomarker Discovery

Coupling large-scale sequencing projects with the amino acid sequence information that can be gleaned from tandem mass spectrometry (MS/MS) has made it much easier to analyze complex mixtures of proteins. The limits of this "shotgun" approach, in which the protein mixture is proteolytically digested before separation, can be further expanded by separating the resulting mixture of peptides prior...

Shotgun proteomics for biomarker discovery in tis - sues

Shotgun proteomics for biomarker discovery in tissues

Proteomics for Cancer Biomarker Discovery

Background: If we are to successfully treat cancer, we must understand the biologic underpinnings in conjunction with early diagnosis. Genome-wide expression studies have advanced the research of many cancers. Nevertheless, understanding which genes are expressed in a tumor is not equivalent to knowing which proteins are being produced. Proteomics hold great promise for careful examination of t...

Proteomics for cancer biomarker discovery.

The emergence of novel technologies allows researchers to facilitate the comprehensive analyses of genomes, transcriptomes, and proteomes in health and disease. The information that is expected from such technologies may soon exert a dramatic change in the pace of cancer research and impact dramatically on the care of cancer patients. These approaches have already demonstrated the power of mole...